ABSTRACT
This study aimed to screen hydrolytic enzyme from ivory coast almond (Terminalia ivorensis C.) and Legumes seeds. The enzyme activity was determined using para-nitrophenyl glucopyranoside (p-NPG) as substrate under basic condition to detect para-nitrophenolate by absorption measuring at 400 nm. Protein concentration was measured by the Bradford method using bovine serum albumin as the standard. Ammonium sulfate precipitation technique was used to separate each crude extract into fractions before activity assay. The enzyme activity was found from three fractions at 0-30% (w/v), 30-60% (w/v) ammonium sulfate of ivory coast almond seeds and another fraction from black gram seeds at 0-30% (w/v) ammonium sulfate precipitated fractions, respectively. The active fractions were further purified by using chromatographic technique.
Keywords: Hydrolytic enzyme, Legumes seeds, Terminalia ivorensis C.