The main objective of this paper is to present and compare different types of approaches and tools applied in the process of developing business networks and clusters in life science. We analyze the effectiveness of some methods of business networks development, identified in the process of business network development in Cluj County. We point out the principles which have been used for developing the SMEGoNET business network for life science small enterprises from Cluj County. Based on experience and knowledge gained from the project, the conclusions focus on reinforcement of transfer of research results, development of scientific and technological culture, transition from project-based organization to more membership-based organization, and highlight the factors that should be taken into consideration when managers decide to become members in a business network.