Organizational trust is the overarching belief that an organization in its communication and behaviors is competent, open and honest, concerned, reliable, and worthy of identification with its goals, norms, and values. Loneliness of employees isn’t a new concept but has received relatively little attention by the researchers and so far its effects on the quality of employees’ life and organizational operations have been proved by limited empirical researches. The purpose of this study is to determine the effect of organizational trust on loneliness in organizations. The feeling of loneliness has been investigated in trust on organizational structure, trust on employees and trust on manager. There is the effect of trust on emotional deprivation and on social isolation. This study examines the relation between organizational trust and loneliness in a firm. To fulfill this purpose, we studied a big scale production firm employing 150 employees in the Bursa, Turkey. The main hypothesis of the study - that “Organizational trust perceptions of employees are negatively related to their level of loneliness at work-life toward the firms.” - seems valid from the results obtained. Furthermore, it has been concluded that there is a significant relationship between organizational trust and loneliness at work-life.